Datasets for transcriptomics, q-proteomics and phenotype microarrays of polyphosphate metabolism mutants from Escherichia coli

Indexación: Scopus.Author acknowledges Fondecyt Grants 1120209, 1121170 and Anillo ACT-1107Here, we provide the dataset associated with our research article on the polyphosphate metabolism entitled, “Multi-level evaluation of Escherichia coli polyphosphate related mutants using global transcriptomic, proteomic and phenomic analyses”. By integrating different omics levels (transcriptome, proteome and phenome), we were able to study Escherichia coli polyphosphate mutant strains (Δppk1, Δppx, and Δppk1-ppx). We have compiled here all datasets from DNA microarrys, q-proteomic (Isotope-Coded Protein Labeling, ICPL) and phenomic (Phenotype microarray) raw data we have obtained in all polyP metabolism mutants.http://www.sciencedirect.com/science/article/pii/S2352340917300860?via%3Dihu

Estudio PRECOST&E: evaluación de los costes constructivos y consumos energéticos derivados de la calificación energética en un edificio de viviendas situado en Madrid

Este artículo presenta un caso de estudio en el que, mediante la calificación energética, se relacionan la eficiencia energética de un edificio, sus costes de construcción y sus consumos energéticos. La variación del comportamiento energético del edificio se realiza a través de cambios en la envolvente y los sistemas de producción de energía. El estudio es teórico y fue realizado en 2009-2010 sobre el proyecto de un edificio residencial de protección oficial situado en Madrid. El trabajo desarrolla una metodología que permite evaluar las variaciones de eficiencia energética en un proyecto y sus im¬plicaciones económicas. Ello permitirá a promotores y arquitectos valorar cómo la adopción de medidas activas y pasivas afecta a la calificación energética de un edificio, a su consumo de energía y a los costes constructivos. Además se analiza la relación entre la orientación de las viviendas y la calificación energética, y las características de los programas utilizados

Global gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosa

Indexación: Web of Science; Scopus.The anuran Rhinella spinulosa is distributed along the Andes Range at altitudes that undergo wide daily and seasonal variation in temperature. One of the populations inhabits geothermal streams, a stable environment that influences life history traits such as the timing of metamorphosis. To investigate whether this population has undergone local adaptation to this unique habitat, we carried out transcriptome analyses in animals from two localities in two developmental stages (prometamorphic and metamorphic) and exposed them to two temperatures (20 and 25 degrees C). RNA-Seq, de novo assembly and annotation defined a transcriptome revealing 194,469 high quality SNPs, with 1,507 genes under positive selection. Comparisons among the experimental conditions yielded 1,593 differentially expressed genes. A bioinformatics search for candidates revealed a total of 70 genes that are highly likely to be implicated in the adaptive response of the population living in a stable environment, compared to those living in an environment with variable temperatures. Most importantly, the population inhabiting the geothermal environment showed decreased transcriptional plasticity and reduced genetic variation compared to its counterpart from the non-stable environment. This analysis will help to advance the understanding of the molecular mechanisms that account for the local adaptation to geothermal streams in anurans.https://www.nature.com/articles/s41598-017-01982-

Role of surface microgeometries on electron escape probability and secondary electron yield of metal surfaces

The influence of microgeometries on the Secondary Electron Yield (SEY) of surfaces is investigated. Laser written structures of different aspect ratio (height to width) on a copper surface tuned the SEY of the surface and reduced its value to less than unity. The aspect ratio of microstructures was methodically controlled by varying the laser parameters. The results obtained corroborate a recent theoretical model of SEY reduction as a function of the aspect ratio of microstructures. Nanostructures - which are formed inside the microstructures during the interaction with the laser beam - provided further reduction in SEY comparable to that obtained in the simulation of structures which were coated with an absorptive layer suppressing secondary electron emission

Cancer Survivors in the United States: A Review of the Literature and a Call to Action

Background: The number of cancer survivors in the U.S. has increased from 3 million in 1971, when the National Cancer Act was enacted, to over 12 million today. Over 70% of children affected by cancer survive more than 10 years, and most are cured. Most cancer survivors are adults, with two-thirds of them 65 years of age or older and two-thirds alive at five years. The most common cancer diagnoses among survivors include breast, prostate and colorectal cancers. This review was conducted to better appreciate the challenges associated with cancer survivors and the opportunities healthcare providers have in making a difference for these patients

Control pid de velocidad de un motor dc manejado comandado mediante joystick

El siguiente proyecto se aplica un control PID de velocidad de un motor DC a través del joystick del Kit AVR Butterfly que opera en cinco direcciones, aquellos movimientos que el usuario realice serán los datos que se enviarán a la entrada del controlador PID, tarjeta POLOLU JRK21V3, y así fijar el sentido de giro y velocidad del motor. Para la implementación del proyecto se utiliza el Kit AVR Butterfly, que posee un microcontrolador ATMega 169, el cual fue programado con el software AVR Studio 4. Se utiliza comunicación inalámbrica entre el Kit AVR Butterfly y la tarjeta POLOLU JRK21V3 mediante los módulos HMTR-434. El joystick envía comandos al módulo HMTR-434 y a su vez se comunica con su par, luego transmite las tramas a la tarjeta POLOLU JRK21v3, la cual alimenta al motor de corriente continua. El motor y la rueda van a estar en conjunto con el POLOLU Encoder ENC01a 0J1216, esta tarjeta tiene dos sensores infrarrojos que van a medir el movimiento de doce dientes a lo largo del aro de la rueda y con esto obtener la señal de realimentación al sistema